1/3/2024 0 Comments Capto q impress pore size![]() The dynamic adsorption capacity of Cellufine MAX Q-hv resin (1.5 mg r-HBsAg/mL of resin) was higher than that for Capto Q (0.9 mg r-HBsAg/mL of resin), with more than 40 % of the chromatographic bed of both resins used is inefficiently. The maximum adsorption capacities of both resins were similar, with values between 1.5 and 1.8 mg of r-HBsAg/mL of gel. The Capto Q resin had an association constant 3.89 times higher than the Cellufine MAX Q-hv resin. Cellufine MAX Q-hv and Capto Q resins were assessed kinetically and thermodynamically, and their the adsorption behavior of r-HBsAg were predicted by Langmuir isotherms with a good adjustment (R 2 > 90 %). This value was statistically different (p 0.05) to that of TMAE anion resin (Merck), currently in use for the industrial production process of the HeberBiovac HB® vaccine. The highest r-HBsAg recoveries were obtained with Capto Q resin (87.16 ± 1.34 %). In this work, the anion exchange chromatographic resins Cellufine MAX Q-hv from JNC Corporation (Japan) and Capto Q from GE Healthcare Life Science (USA) were evaluated for the purification process of the recombinant hepatitis B surface antigen (r-HBsAg), as active pharmaceutical ingredient. ![]() Calle 114 No 11901 entre Ciclovía y Rotonda, CP 19390, La Habana, Cuba.Ī thermodynamic and kinetic understanding of chromatographic separation is required for the design of the chromatographic process’ operating system. Pedro Domínguez Aldás 1, Lourdes Zumalacárregui de Cárdenas 2, Abel Caballero 1ġ Centro de Ingeniería Genética y Biotecnología, Ave 31 e/ 158 y 190, Cubanacán, Playa, CP 11600, La Habana, Cuba.Ģ Facultad de Ingeniería Química, Universidad Tecnológica de La Habana José Antonio Echeverría. The multimodal property of the ligands in the core ensures strong binding with most impurities over a wide range of pH and salt concentrations.Assessment of the chromatographic resins Cellufine MAX Q-hv and Capto Q for r-HBsAg purificationĮvaluación de las resinas cromatográficas Cellufine MAX Q-hv y Capto Q para la purificación del r-HBsAg The core of each bead is functionalized with ligands that are both hydrophobic and positively charged, resulting in a highly efficient multimodal binding of the various contaminants small enough to enter into the core.
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